[PDF] – e. e ulate theory states that (1) inherited characters are deter~ e 4. Distinguish between an organism\\\’s phenotype and. e e – Free Download PDF

– e. e ulate theory states that (1) inherited characters are deter~ e 4. Distinguish between an organism\’s phenotype and. e e

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1 Gntics Th Principls of Mndl Objctivs By th nd of this xrcis you should b abl to: 1. Dscrib simpl gntic dominanc, incom…

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Genetics The Principles of Mendel

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Objectives By the end of this exercise you should be able to : 1. Describe bare genetic dominance, incomplete authority, and deadly inheritance. 2. Describe possible genotypes for some of your

personal traits inherited as dominant and recessionary

genes.

3. Explain the importance of Mendel ‘s Law of Segre~

gation and Law of Independent Assortment.

4. Distinguish between an organism ‘s phenotype and

genotype.

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ublished papers are the chief means of communicating scientific discoveries. One of the most celebrated of these papers, entitled “ Experiments in Plant Hybridization, ” was written in 1866 by Gregor Mendel, an austrian monk. Al~ though this newspaper late became the basis for genetics and in~ heritance, it went largely unnoticed until it was rediscovered independently by several european scientists in 1900. The experiments and conclusions in Mendel ‘s paper now form the foundation of mendelian genetics, the topic of today ‘s exercise. Mendel ‘s greatest contribution was to replace the blend~

ing hypothesis of inheritance, which stated that all traits blend

with each other, with the particulate theory. Mendel ‘s partic~

ulate theory states that ( 1 ) inherited characters are deter~

mined by particular factors ( nowadays called genes ), ( 2 ) these

factors occur in pairs ( i.e., genes occur on enate and pater~

nal homologous chromosomes ), and ( 3 ) when gametes form,

these genes segregate sol that lone one of the homologous copulate

is contained in a particular gamete. Recall from Exercise 15

( Meiosis ) that each gamete has an equal chance of possessing either member of a pair of homologous chromosomes. This region of the particulate hypothesis is jointly known as Mendel ‘s First Law, or the Law of Segregation. Mendel ‘s Second Law, or the Law of Independent Assortment, states that genes on nonhomologous or unlike chromosomes will be distributed randomly into gametes ( fig 17.1 ).

Before you start this exert, concisely review in your textbook some principles and terms pertinent to today ‘s drill. A gene is a unit of heredity on a chromosome. A gene has alternate states called alleles, contributed to an or~ ganism by its parents. Alleles for a detail gene occur in pairs. Alleles that mask formula of other alleles but are themselves expressed are dominant ; these alleles are normally designated by a capital letter ( for example, P ). Alleles whose formulation is masked by dominant allele alleles are recessive, and they are designated by a small letter letter ( for exercise, phosphorus ). The genotype of an organism includes all the alleles present in the cell, whether they are dominant allele or recessive. The physical ap~ pearance of the trait is the phenotype. frankincense, if imperial flowers ( P ) are dominant to white flowers ( phosphorus ), a establish with purple flowers can have a genotype PP or Pp. A implant with white flowers can only have a genotype pp. When the mated alleles are identical ( PP or pp ), the genotype is homozygous. Het, erozygous refers to a copulate of unlike ( Pp ) alleles. With this minimal review, you ‘re cook to apply this information to solve some genetics problems.

SIMPLE DOMINANCE Assume that purple flowers are dominant to white flowers. If a homozygous purple~flowered plant is crossed ( mated ) with a homozygous white~flowered plant, what will be the phe~ notype ( physical appearance ) and genotype of the young ? Parents :

Gametes :

Offspring :

PP ( homozygous dominant = purple flowers ) X pp ( homozygous recessive = egg white flowers ) P from the

purple~flowered

phosphorus from the

white~flowered

parent parent

genotype = Pp phenotype = purple flowers

This beginning generation of offspring is called the first filialorF 1 genesis ( figure. 17.2 ). Each of the Fl young can produce two possible ga~ metes, P and p. Mendel noted that the gametes from each of

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Paternal gamete

Maternal gamete

….., .. — — homologous pairs

Diploid offspring

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Potential gametes

figure 17.1 Independent assortment increases genic unevenness. Independent assortment contributes new gene combinations to the following generation because the or ientation of chromosomes on the metaphase pla tellurium is random. For exemplar, in cells with three chromosome pairs, eight different gametes ca newton result, eac h with different comb inations of parental chromosomes.

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P

P

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P

P

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White parent pp

• Pp

P

1 pp

1 ,. p+ p= pp.

9c ! ‘

2. P+p=Pp.

P

P Pp

1

9d ‘

P

p

~ P- ” PP

Pp F1 generation

p- ” pP

pp

3. p+P= pP.

P

pP

pp

4. P + P = PP.

calculate 17.2 In Mendel ‘s cross of purp lupus erythematosus by white flowers, the original parents each merely make one type of gamete. The resulting F [ genus tion are all Pp heterozygo tes with purple flowers. These F j then each make two types of gametes that can be combined to produce three kinds of F2 young : PP homozygotes ( purple flowers ) ; Pp heterozygotes ( besides purple flowers ) ; and pp homozygotes ( white flowers ). The proportion of prevailing to recessive pheno types is 3 : 1. The scab io of genotypes is 1:2 : 1 ( 1 PP : 2 Pp : 1 pp ).

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E XERCIS E 17

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F2 generation 3 purple : 1 White ( 1 PP:2Pp:1pp )

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TABLE

SAFETY FIRST Before coming to lab, you were asked to read this drill then you would know what to do and be aware of safety issues. In the space below, briefly list the condom issues associated

17.1

RESULTS OF COIN-FLiPPING EXPERIMENT SIMULATINC RANDOM MATING OF HETEROZYGOUS

( PI ‘ )

INDIVIDUALS

Response Heads- heads

with today ‘s procedures. If you have questions about these issues, contact your testing ground assis­

Number

tant before starting work.

= PP = purple flowers

Heads-ta ils = PI ) = purple florida owers Ta ils-tails = PI ) = white flowers

Procedure 1 7. 1

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the parents combine with each other randomly. frankincense, you can simulate the random entangle of gametes from the F [ generation by flipping two coins simultaneously. Assume that heads designates the purple-flower allele ( P ), and tails designates the white-flower allele ( phosphorus ). Flipping one coin will determine the character of gamete from one parent and flipping the other will determine the gamete from the other rear. To demonstrate this technique, flick two coins simultane­ ously 64 times and record the occurrence of each of the three possible combinations in table 17.1.

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Question 1 What is the proportion of purple-flowered ( PP or Pp ) flowered ( pp ) offspring ?

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Genotype of children ‘s founder Possible gametes of mother

white­ possible offspring

Phenotypic ratio of children

Parents : Gametes : offspring :

Pp

X

Pp

( P or phosphorus ) adam ( P or p ) PP Pp pP pp ‘-v — ‘ ‘-v — ‘

procedure 1 7 .2 Determine color and height ratios for corn whiskey plants

Color of grains ( karyopses ) and acme of Zea ma ) ‘s ( corn )

plants are frequently determined by a one gene.

1.

Examine ( a ) the ears of corn having red and yellow grains and ( boron ) the tray of tall and gnome plants on demonstration.

2.

Record your observations here and determine the probable genotypes of the parents of each cross.

3 empurpled 1 blank thus, the theoretical genotypical proportion for the offspring of the F ) generation is 1 PP : 2 Pp : 1 pp, and the phenotypical ratio is 3 purple : 1 white.

Probable genotypes of parents :

Question 2 a. How do these ratios compare with your data derived from mint flipping ?

Color

of Corn Grains

Number of red grains Number of white grains Ratio of crimson : ashen grains

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Genotype of children ‘s mother – – – ­

Possible gametes of beget to

Keep these results in mind and return to the original prob­ lunar excursion module : What are the genotypes and phenotypes of the off­ give of the F J generation ?

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Albinos are homozygous recessive for the couple of alleles that produce pigments of skin, haircloth, and eyes. Suppose a woman having convention colored hide and an albino beget marries an albino man. Record the genotypical and phenotypical ratios of their children.

Genotypic proportion of children

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Determine genotypical and phenotypical ratios for albinism

Probable genotypes of parents _ _ _ X _ __

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Would you have expected a closer similarity if you had flipped the coins 64,000 times alternatively of 64 times ? Why or why not ?

Height

of Plants

Number of tall plants Number of dwarf plants Ratio of improbable : dwarf plants probable genotypes of parents ____ X ______

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Genctic.~

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3.

The precede crosses involved entirely one trait and thus are term monohybrid crosses. Let ‘s now examine a cross involving two traits ; that is, a dihybrid crisscross. Your teacher will review with you the basis for working genetics problems involving dihybrid crosses.

In corn, red ( R ) seed color is dominant to flannel ( r ) seed color, and smoothness ( S ) is dominant allele to wrinkled ( s ) source. Observe the hazelnut of corn derived from a hybridization between parents having genotypes RRSS and rrss. question 3 a. What is the expect genotype for the F ) generation ?

b.

Will all Fl offspring ( seeds ) have the lapp genotype ?

interrogate 4 a. What are the predict genotypes for the FI ( i.e., second base ) generation ?

INCOMPLETE DOMINANCE Some traits such as flower color are controlled by incom­ plete dominance. In this type of inheritance, the heterozy­ gous genotype results in an intermediate feature. For model, if a plant with bolshevik flowers ( RR ) is crossed with a establish having white flowers ( rr ), all of the young in the first filial ( F 1 ) coevals will have pink flowers ( Rr ). Parents : Gametes : offspring :

Question 6 What are the have a bun in the oven ratios of loss, pink, and ashen flowers in a traverse involving two pink-flowered parents ?

LETHAL INHERITANCE Lethal inheritance involves inheriting a gene that kills the young. Observe the tray of green and albino seedlings of corn. The albino plants can not photosynthesize and there­ bow die deoxyadenosine monophosphate soon as their food reserves are exhausted. question 7 a. What is the ratio of green to albino seedlings ?

b.

boron.

In what proportion will they occur ?

4.

To test your prediction in Question 4 count the number of kernels for five rows for each of the postdate phenotypes : Red, fluent Red, wrinkled White, smooth White, wrinkled

Question 5 a. What are the genotypes of the F ) genesis ?

RR ( red ) X rr ( white ) RX radius Rr ( pink )

Based on this proportion, what might you expect were the genotypes of the parents ?

question 8 Why is it impossible to cross a k and an albino plant ?

OTHER SOURCES OF GENETIC DIVERSITY Genetic diverseness can besides result from multiple alleles, gene interactions ( hypostasis ), continuous variation, pleiotropy, environmental effects, linkage, and sex linkage. Although clock limitations prohibit exercises about these topics, be certain to review them in your textbook.

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How did your data compare with those that you predicted ?

EXERCISE 1 7

Blood character of humans provides an excellent exercise of codominance, another type of mendelian inheritance. In codominance, both alleles contribute to the phenotype of a heterozygote. For example, all individuals have one of four blood types : A, B, AB, and 0 ( fig. 17.3 ). These blood groups are determined by the presence of compounds called

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Possible alleles from female

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Blood types and agglutination

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Recip’ient ( serum type ) A

B

AB

0

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+

G G

+

+

+

0

+

0

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Blood types

IAS I [ §J [ QJ

( a )

e+ ==

( c )

( bel )

Agglutination No reaction

Figure 17.3 ABO blood groups. ( a ) Multiple alleles control the ABO blood groups. different combinations of the three I gene alleles result in four different blood type phenotypes : type A ( either IAI A homozygo tes or IAj heterozygotes ), type B ( either IBIB homozygo tes or IBj heterozygotes ), type AB ( lAIB heterozygotes ), and type 0 ( jemaah islamiyah homozygotes ). ( b ) The blood agglutination reaction. Agglutination occurs when blood cells stick and clump together. ( c ) Agglutination will occur when donor blood cells are incompatible with recipient role serum, as designated by a +.

TARLE

17.2

CHARACTERISTICS OF INDIVIDUALS WITH THE

F

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